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Background: Over half of women of reproductive age are now overweight or obese. The impact of maternal high-fat diet HFD is emerging as an important factor in the development and health of musculoskeletal tissues in offspring, however there is a paucity of evidence examining its effects on tendon. Alterations in the early life environment during critical periods of tendon growth therefore have the potential to influence tendon health that cross the lifespan. We hypothesised that a maternal HFD would alter biomechanical, morphological and gene expression profiles of adult offspring rotator cuff tendon. Eight female and male offspring from each maternal diet group were weaned onto a standard chow diet and then culled at postnatal day for tissue collection. Supraspinatus tendons were used for mechanical testing and histological assessment cellularity, fibre organisation, nuclei shape and tail tendons were collected for gene expression analysis.
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